Sentence examples for insert vector from inspiring English sources

DNA fragments 1.6 1.8 kb in size were ligated into the pLEXX AK double-insert vector and transformed into electrocompetent E. coli cells as directed by the manufacturer (Cloneplex AK kit, Lucigen Inc .. Purified plasmids were sequenced with each of the four primers from the pLEXX AK double insert vector.

To generate compatible end for the 35S insert vector SUB 3×myc pCAMBIA2300 was digested first with BamHI, made blunt with T4 DNA polymerase, and subsequently gel purified and digested with SpeI generating 35S::SUB:35Syc pCAMBIA235S.

Ligation was carried out using T4 DNA ligase at an insert: vector molar ratio of 3 1.

Sixty ng of PCR product was used for each cloning reaction, maintaining a molar ratio insert : vector of 3 : 1.

We also observed a high cloning efficiency (at the optimal insert:vector ratio) regardless of the purification steps used.

Annealed oligos were ligated into pSM30 vector using Rapid Ligation Kit (Roche) with 15:1 insert:vector ratio.

Therefore, future work should involve the use of large insert vectors to explore adjacent stretches of genomic DNA in order to rule out this possibility.

Consequently, the inserted vector backbone is flanked by LE* and RE* sites and can be rescued by Cre.

PCR-base genome walking of Ch-TL2 genomic DNA successfully elucidated the integration sites and flanking regions of the inserted vector cassette.

where e ̃ i k is an NPK × 1 zero inserted vector formed by e ̄ i k with a (k - 1) chip initial offset.

The full-length p53 coding region (1179 bp) was PCR-amplified from the pRK-7-Flag-Neo-Insert vector (a generous gift from Dr. Shu hongbin (Wuhan University), which contains the full length of p53 coding sequence, using the p53-1 primer (5'-CAGGGATCCATGGAGGAGCCGCAGTCAG-3') and the p53-2 primer (5'-AGAGAATTCGTCTGAGTCAGGCCCTTCT-3').