Sentence examples for incorporated vector from inspiring English sources

PCR primers also incorporated vector sequence, restriction enzyme sites used for cloning (NcoI at 5' and XhoI 3') and gene specific sequence.

While, large indels could not be analyzed due to the lack of PCR amplification, analysis of short indels showed that DSB sites frequently incorporated vector DNA sequences, while one line contained rice mitochondrial DNA (data not shown).

We incorporated improved vector designs so as to minimize the consequences of random integration, facilitate the removal of potential antigens, and avoid unwanted exon skipping.

Therefore, we newly designed an Arg-Gly-Asp (RGD) peptide-incorporated adenovirus vector expressing cytoprotective heme oxygenase-1 (HO1) gene (RGD-Adv-HO1) for effective gene therapy to islets via RGD integrin interaction between adenovirus and islet.

The peak immune response was observed with the capsid incorporated MPER vectors at 54 d.p.p, which also corresponds to 14 days post-boost (d.p.b).

These fragments were incorporated into vectors for expression of EGFP from the hCMV promoter as indicated in Figure 1B.

To construct the plasmid containing Flag-tagged full length Usa1 or various deletions, USA1 gene was amplified by PCR from wild type or USA1 deletion strains and incorporated into pRS416Gal1 vector containing Flag epitope at C-terminus.

The resulting DNA was digested using XbaI and XhoI and incorporated into expression vector pj214 from DNA2.0.

Lentiviral vectors can also be pseudotyped with engineered GP64 proteins, decay accelerating factor was fused to the amino terminus of GP64 and incorporated into lentiviral vector particles [ 24].

Transcription templates for monocistronic RNAs were created using PCR containing a template-specific forward primer with a T7 promoter incorporated and a vector specific reverse primer.

IN-fusion proteins thus become incorporated into new vector particles as a part of the large Gag-Pol polyprotein, and they are released from Pol only after the vector particle has left the producer cell, thereby enabling the packaging of both toxic and inert proteins into LVVs and VLPs.