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GFP fluorescence was investigated 3 days after infiltration in excised leaf sections that included the infiltrated zone and 1-3 cm of surrounding non-infiltrated tissue, using an Olympus BX-60 upright Fluorescence Microscope and MF filter.
Twenty to 30 leaf pieces covering the infiltrated zone from each of the treatments were harvested 12 h after infiltration.
Correspondingly, the following three different types of filter cakes were observed: a filter cake, a filter cake with an infiltrated zone, and infiltrated zone without filter cake.
Quantitative GUS assays were carried out on the infiltrated zone.
Taken together, this indicates that the expression of the flagellin gene, fla Lso, causes necrotic cell death in the infiltrated zone of N. benthamiana.
Neither peptide induced cell death in the infiltrated zone but callose deposition was observed in infiltrated N. benthamiana, tomato and potato, with more callose deposition from flg22 Lso compared to flg22 Las in both tobacco and tomato.
Similar(53)
An inner area is composed of spaced infiltrated cells (Zone B) and dense cellular infiltrate (zone C) surrounding the worm (Figure 1A).
In contrast, nearly all tumor cells and microvessel endothelial cells (arrows in Fig. 3b) in the infiltrating zone were absent of PDCD10 immuno-signal (green) but showed strong PCNA immunoreactivity (red) (Fig. 3b) and appeared negative for caspase 3 staining (Fig. 3c and e).
Chemokines released from epithelial cells can recruit neutrophils and a modest number of monocytes which in turn can infiltrate submucosal zone within 24 48 hours after initiation of the infection [ 3].
The cells infiltrated the damaged zone left by the simulated heart attack and meshed with other cardiomyocytes physically and electrically, allowing them to beat.
The HV had infiltrated the pink zone along three routes via Nos Kalik, across the Čikola river and by boat sailing upstream along the Krka River, during the night of 20/21 June.
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