Sentence examples for infiltrated area from inspiring English sources
The comparison of pTRA_35STX and pTRA_e35STX indicated that the additional enhancer sequence in pTRA_e35STX has a significant impact on recombinant protein accumulation, resulting in the appearance of necrotic lesions in the infiltrated area by 6 days post-infiltration.
Three types of area were observed (fig. S2A): an infiltrated area (that display necrosis and macrophage infiltrate) (fig. S2B&2C), a preserved area (normal histology) (fig. S2D&2E) and a necrotic non-infiltrated area (that display necrosis without macrophage infiltrate) (fig. S2F&2G).
Leaf necrosis was observed in the infiltrated area 4 or 5 days post infiltration (dpi) in plants for all constructs, with cytosol-targeted construct causing the most severe symptoms (data not shown).
Unlike seedlings, leaves of adult Arabidopsis plants infiltrated with B. subtilis did show tissue damage limited to the infiltrated area.
Two to three days post infiltration, leaf disks (1.2 cm diameter) were punched from the infiltrated area and directly observed with a LSM 510 Meta Zeiss upright laser scanning confocal microscope (Objective C-Apochromat 40X/1,2 water, 488 nm laser and 505-530 band-pass filtor to GFP, 543 nm laser and 585-615 band-pass filtor to mCherry).
When comparing the cells suspension results to the disease symptoms evolved in the leaves after infiltration of the mutants, it can be seen that S. Typhimurium WT and its ΔrfaH mutant did not promote any observed signs following infiltration to leaves whereas ΔinvA mutant induced mild local necrosis of the infiltrated area in the leaves.
When bronze-coloured ANT1 leaves were infiltrated with IFS/CHI, the infiltrated areas were less red-coloured after 7 d compared with the surrounding uninfiltrated areas or the areas infiltrated with buffer only (Fig. 5).
Three days after infiltration, DsRed2 was detectable in infiltrated areas as bright fluorescence signals in the cytoplasm and nucleoplasm, indicating successful transfer and expression of the reporter gene from A. tumefaciens to the plant (Additional file 1: Figure S1C).
Transient expression of either SlMKK2 DD or SlMKK4 DD resulted in a typical and strong HR and significant accumulation of H2O2 in the infiltrated areas of N. benthamiana leaves 48 hr after infiltration, indicating that an activated phosphorylation status of SlMKK2 and SlMKK4 is necessary for their biochemical functions.
However, when these bacteria were infiltrated at lower concentrations (105 and 106 cfu/ml) despite the fact that the infiltrated areas were similar, canker lesions (brown spots in Figure 2B) produced by ΔXacFhaB appeared in a more expanded manner than typical lesions observed with ΔXacFhaC or wild type bacteria (Figure 2B, left leaf).
CymRSV p19 infiltration of tobacco cultivars I64 and 81V9 did not cause necrosis in the infiltrated areas, differing from a recent report where p19 from TBSV- induced necrosis in several tobacco cultivars including cv.
