The color signal was displayed by HRP substrate reagents (optEIA, BD Bioscience, San Diego, CA).
The proteins were visualized by Immobilon Western Chemiluminescent HRP Substrate (Millipore, WBKLS0100).
Changing the color of horseradish peroxidase (HRP) substrate to green indicates a positive result.
Chemiluminescent HRP substrate was from Pierce.
The individual phage ELISA kinetics were followed and quantified colorimetrically using soluble HRP substrate (ABTS/H2O2).
Antibody binding was visualized with an Immobilon Western Chemiluminescent HRP substrate (Millipore #WBKLS0100).
The addition of chemiluminescent HRP substrate solution (Millipore, USA) was used to develop the images.
HRP substrate was added and color development was stopped with 1N NaOH after 30 minutes incubation.
The blot was developed using the Western Chemiluminescent HRP substrate (Immobilon).
Reaction was developed with the HRP substrate tetramethyl benzidine (TMB) (Becton-Dickinson).
Following immunolabeling, membranes were washed and reacted with Super Signal chemiluminescence HRP substrate (Pierce Chemical Co).
