Sentence examples for hrp used from inspiring English sources

Secondary antibodies included a goat anti-mouse (or rabbit) antibody conjugated to AlexaFluor 568/594 (use at 0.02 µg/ml) (or HRP used at 1 µg/ml for western blotting) (Invitrogen).

We therefore doubt that Lyc e 2 is a true allergen, albeit inducing histamine release [7], [16] that appears to result from application of a single plant glycoprotein in high concentration, similar to HRP used as control in our BAT assays.

Secondary antibodies were anti-mouse HRP, used at a dilution of 1 2500 (from GE Healthcare, Little Chalfont, UK).

At this concentration there was no effect of residual EDTA on either ECAO or the HRP used in the coupled assay.

Secondary antibodies conjugated with fluorochromes (used at 1 500 dilution) or HRP (used at 1 5000 dilution) were from Invitrogen Milann, IT) or Santa Cruz Biotechnology Santaa Cruz, CA, USA).

Bound proteins were analyzed by SDS-PAGE and western blotting with anti-Myc mouse monoclonal antibody 9E10 (Santa Cruz Biotechnology) used at 1 5000, followed by Rabbit anti Mouse HRP used at 1 8000.

TMB can be converted into a colored product when oxidized by HRP using hydrogen peroxide as the oxidizing agent.

Native HRP (nHRP) and a nonglycosylated recombinant form containing a six-histidine tag at the C-terminus, CHisrHRP, produced by genetic engineering of nonglycosylated recombinant HRP using an E. coli expression system, have been used for adsorptive modification of Au electrodes.

BiP and UFBP1 antibodies were conjugated to HRP using the 'lightning-link HRP conjugating' kit (Innova Biosciences) for detection after immunoprecipitation.

After transfer of proteins to polyvinylidene fluoride (PVDF) membranes, biotinylated proteins were detected with streptavidin-horse radish peroxidase (HRP) using chemiluminescence (ECL detection system, Amersham; Pittsburgh, PA).

Each purified immunoglobulin was covalently labeled with horseradish peroxidase (HRP) using EZLink Peroxidase (Pierce) according to the manufacturer's instructions.