Sentence examples for hrp type from inspiring English sources

Polyoxyethylene (5) isooctylphenyl ether (Igepal CA-520), HRP type VI-A (HRP), 3,3′5,5′-tetramethylbenzidine (TMB), citric acid, dimethyl sulfoxide (DMSO), and rhodamine B isothiocyanate (RITC) were purchased from Sigma-Aldrich.

We explored the possibility that Pst DC3000 delivers AvrRpt2 protein to plant cells via the hrp (type III) secretion pathway.

Four weeks after implantation, rats were placed under deep anesthesia, and 50 mg/ml of HRP (type 11, molecular weight 40,000 Da, Sigma-Aldrich) was instilled into the oral mucosa surrounding the implant for 60 min.

In brief, H2O2 oxidizes leuco crystal violet (LCV) (Spectrum) in the presence of the enzyme horseradish peroxidase (HRP type II) (Sigma) forming a crystal violet cation, which has an absorbance maximum around 590 nm [40].

Leuco crystal violet (Spectrum) in the presence of the enzyme horseradish peroxidase (HRP) type II (Aldrich) forms a crystal violet cation, which has an absorbance maximum at 590 nm.

HRP type VI (0.92 mg) is dissolved in 200 μL of 50-mM phosphate buffered saline (PBS, pH 7.4) and incubated for 1 h at 37°C with sulfo-succinimidyl-4- N-maleimido-methyl cyclohexane-1-carboxylate (sulfo-succinimidyl-4- N-maleimido-methyl cyclohexane-1-carboxylate sulfo-succinimidyl-4- N-maleimido-methyl cyclohexane-1-carboxylate).

The role of the hrp-Type Three secretion System (T3SS) in infection and bacterial transmission to seed has been previously demonstrated [ 17].

Native HRP, wild type recombinant HRP (rec-HRP) and its two mutant forms containing a six-histidine tag at the C- or N-terminus, CHisrec-HRP and NHisrec-HRP, respectively, have been used for an adsorptive modification of the gold electrodes.

Progress in the genetic and biochemical dissection of the hrp-encoded type III secretion pathway has revealed new mechanisms by which phytopathogenic bacteria infect plants.

Furthermore, Klement and collaborators [45] demonstrated that induction of local induced resistance (IR) prior to infiltration of P. syringae can suppress expression of the hrp-encoded type III secretion system (TTSS), which is known to be involved in both pathogenesis and HR elicitation.

For example, in a recent study by Delamarre et al. (13) it was demonstrated that a heme-free form of horseradish peroxidase (apo-HRP) was more sensitive to lysosomal proteolysis than wild-type, heme-containing HRP (wt-HRP) in vitro.