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Then, the optimization of fermentation conditions for SrtA production was manipulated by the combination of traditional one-factor-at-a-time approach and response surface methodology (RSM) at shake-flask stage, which is an effective and simplified method that has gained great success in the production of recombinant proteins (Papaneophytou and Kontopidis 2014).
Previous studies have confirmed the feasibility of using the 2A site for the expression of multiple transgenes [24], [25], and 2A-based polycistronic expression has gained great success in generating induced pluripotent stem cells that require four factors working together [20], [21], [26] [28].
Although site-specific detection for O-GlcNAc peptides with ETD has gained great success, its utility for the O-GlcNAc site mapping on intact individual protein(s) (i.e., the 'top-down' approach) is still to be explored.
Eli Broad NET WORTH: $5.8 BILLION "Those of us who have gained great success have an obligation to pay more taxes.
"Those of us who have gained great success have an obligation to pay more taxes," he told Forbes in 2011.
Large-scale solar heating plants for district heating networks have gained great success in Europe, particularly in Denmark.
Among the popular methods for modeling TFBS, position-specific weight matrix (PWM) [ 1, 2] and k-mer based approaches [ 3, 4] have gained great success [ 5, 6].
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MA of Applied Linguistic, Maquarie University, Australia