We have generated 574 Mbp of Illumina/Solexa sequences from barley total genomic DNA, representing about 10% of a genome equivalent.
We also conducted real-time PCR for β-globin (110 bp) to determine the genome equivalent present in each sample and to normalize samples for genomic DNA content.
This level of genome equivalent coverage affords a 99.88% chance that a particular genomic sequence of interest will be found at least once in a library [see 39].
However, the manufacturer's verification documentation demonstrates detection at even 1 genome equivalent, though less than 100% (data not included).
Positive classification of standards nominally containing a single genome equivalent was demonstrated, and no false positives were reported.
Estimated detection limits in reagent-grade water were five genome equivalent copies (GEC) per reaction and two to four rotavirus particles per reaction.
Then, the concentration of P. putida KT2440 DNA was converted to a genome equivalent for calculation of copy numbers in the real-time PCR assays (Cottyn et al. 2011).
Viral genome equivalent (vge) titers were determined by dot blot hybridization as previously described [45], [46].
In total, 6.7 x genome equivalent BAC clones were screened for this study (Table 4).
The maximum titres we recorded in the ovaries of infected queens were about 1012 genome equivalent copies per ovary for DWV and 1013 genome equivalent copies for VDV-1.
Based on the published genome of Copenhageni, one genome equivalent corresponds to 5.1 10−15 g DNA [39].
