Don't let that slick, cool exterior fool you, the gel running sparkling through his jet black hair, the hint of a nick in his eyebrows, the sharp, slim Wild West suit.
The restriction enzyme digests were separated on a 1 % agarose gel running in 1× TAE buffer at 100 V for approximately 1 h.
Furthermore, due to the small amount of moisturizing agent employed, the risk of conductive bridges between adjacent electrodes due to gel running is considerably reduced.
At the very least, some of you might save a few valuable gel running hours and perhaps even learn to avoid stepping where I shouldn't have.
The resulting digestion products were resolved by agarose gel electrophoresis using 1.5% agarose (w/v) gel running for 90 min at 70 V and the gel was viewed using Alpha Imager HP Gel-documentation system (Cell bioscience, USA).
As time goes on, clear "work triangles" within the lab begin evolve where tools specific to a task (e.g. gel running, staining, and visualization) are all located in a consolidated space.
