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Exact(4)
Nested PCR was required for one of the FLS lines (RA4 non-erosion FLS line).
The patterns of differential methylation of each individual FLS line were very similar regardless of passage number.
Each FLS line from an individual OA patient was used for each experiment, which was repeated two times with different OA FLS lines.
Most of the mutations were identified in a single subclone, although multiple copies of one mutation in codon 321 AAA to GAA were observed in an erosion FLS line.
Similar(55)
AtHMA4 FL line 2 showed low amplification levels for AtHMA4 but actin was also low in this sample.
To test whether NCad was responsible for junction assembly and contact inhibition of cell growth in these cells, NCad expression was inhibited in the IC2 FL line using siRNA.
Silique length and also seed number per silique were both slightly but significantly smaller than wt in both AtHMA4-FL lines (Figure S3).
To test whether ICAM-2 controls endothelial barrier function, we measured transendothelial electrical resistance (TEER) by impedance spectroscopy in IC2 neg and IC2 FL lines.
Mice carrying Trp53 fl allele, Nestin Cre and hGFAP Cre transgenes were from Jackson Laboratories (Bar Harbor, ME), stocks # 008462, 003771, and 004600, respectively, Strains were backcrossed to C57BL/6J (Nestin Cre, Rest GTi and Trp53 fl lines) and to FVB background (hGFAP Cre line) for at least 10 generations.
Subjects were chosen to participate based upon four key determinants: the location of their residence (38% urban, 42% suburban, and 20% rural), whether the residence incorporated an integral garage (IG; IG = 16%; non-IG = 84%), the proximity of the residence to a major road [coded as first line (FL); FL = 44%, non-FL = 56%], and whether they were exposed to ETS (ETS = 32%; non-ETS = 68%).
In contrast, the R8-conjugated PNA2-Fl (blue line) is efficiently internalised.
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