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However, no enhancer activity was noted in the FLS cell line SW982 and cultured RA FLS (Fig. 2b).
To determine the effects of KIAA1199 expression on FLS cell proliferation, the MTT assay was performed on FLS cells treated with scrambled siRNA, KIAA1199 siRNA duplex, pcDNA3.2DEST and pcDNA3.2DEST-KIAA1199 for 24 h, 48 h, 72 h and 96 h at 37°C.
To examine whether the life span of fibroblast-like synoviocytes (FLSs) can be extended and to establish FLS cell lines that preserve the characteristics of primary FLSs, we introduced human catalytic subunit of telomerase (hTERT) gene into human osteoarthritic (OA) FLSs.
In contrast, IL-17 derived from Th17 cells has not been reported to modulate RA FLS cell growth.
We recently observed expression of synoviolin in T cells, macrophages and RA FLS, which is increased by the predominantly macrophage-derived cytokines IL-1 and TNF known to regulate RA FLS cell growth and survival [3].
Three RA FLS cell lines were tested in all.
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The FLS cell-line showed an initial transient response to sphingosine which may be explained by TRPV4 channels but was not observed in FLSs from patients.
In this study we investigated TRPM3 and its stimulators by using a model FLS cell-line and primary cultures of FLSs from patients diagnosed with RA.
Intracellular calcium measurement detected channel activity in a FLS cell-line and primary cultures of FLSs from patients with rheumatoid arthritis.
HIG-82 cells are a fibroblast-like synoviocyte (FLS) cell-line from soft tissue lining the knee joints of rabbits [ 20, 3].
Because of limited supply of synovial biopsies we first sought to investigate the functional relevance of TRPM3 expression through experiments on a FLS cell-line (HIG-82 cells), a proposed model system of proliferating FLSs in joints.
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