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The dysfunction of the serotonergic system in the FSL line is also documented by the increased tissue levels of 5-HT and 5-HIAA, that can be normalized by chronic antidepressant treatment [5], [7], [33].
Significantly increased expression of the serotonin receptor 1a (Htr1a) in P/FC (Table 1) and the serotonin receptor 2a (Htr2a) in HIP (Table 2) was observed in the FSL line compared to the FRL line.
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Both cohorts of animals showed significant differences in response between FRL and FSL lines, FSL rats being significantly more immobile than FRL rats (immobility time: cohort 1: FSL 160.3±15.6 s; FRL 98.1±14.5s, ANOVA F 1,19) = 8.31, p-value for: 0.0095; cohort 2 FSL 99.8±23.1s; FRL: 41.0±7.9s, ANOVA F 1,15) = 6.37, p-value 0.023).
Antidepressant effects of acute or chronic nicotine treatments in swim test immobility of Flinders sensitive line (FSL) rats, an animal model of depression, were recently demonstrated (Tizabi et al. Psychopharmacology 142 193, 1999).
Saccharin and ethanol intakes were measured in seven strains of rats known to differ in their preferences for ethanol: The Fawn-Hooded (FH), alcohol-preferring (P) and Maudsley Reactive rats have been reported to drink ethanol voluntarily, whereas the alcohol-nonpreferring, Maudsley Nonreactive and Flinders Line (FSL and FRL) rats do not.
The Xilinx FSL link interface includes built in FIFOs on each channel.
Prepare the working FSL solution by diluting stock FSL-biotin solution in buffer (as above).
Using the tool FSL-view of FSL software and Talairach Demon Labels (http://www.fmrib.ox.ac.uk/fsl), the anatomic location of significant clusters was detected.
These data clearly confirm the robustness of the microarray data, and show that dramatic gene expression differences exist between the FSL and FRL rat lines.
Note: FSL constructs may become internalised by some cell types over time.
It was numerically demonstrated that the FSL can resolve 40 nm lines with a 30 nm gap at 376 nm wavelength (shown in Fig. 6 (d)) [73].
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