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RPTP-AP fusion proteins were obtained from baculovirus infected cell supernatants generated by the Caltech Protein Expression Center.
The isolated RNA was submitted to the Gene Expression Center, University of Wisconsin-Madison where a quality control test was performed on the RNA samples.
Stained U133 Plus 2.0 arrays were scanned at 3 µm resolution using the Affymetrix GeneChip Scanner 3000 at the Gene Expression Center (University of Iowa, Iowa City, IA).
We thank Davin Townley-Tilson and Yvette Chuang for making the mPAP-Tr-H6 clone, Yvette Chuang for excellent technical assistance, Jost Vielmetter and Michael Anaya at the Caltech Protein Expression Center for purifying mPAP protein and for providing the western blot data shown in Fig. 1D.
We thank Yvette Chuang for excellent technical assistance, Nick Moss for providing rats, Pirkko Vihko for providing a rat TM-PAP plasmid, Jost Vielmetter and Michael Anaya at the Caltech Protein Expression Center for purifying mPAP protein and Aves Labs for making our chicken anti-PAP antibody commercially available.
Both ESC and ESC-derived neurons were processed for ChIP with mouse monoclonal anti-NRSF antibody (Protein Expression Center, Caltech).
Similar(49)
Our initial criteria for assessing expression centered on using 'present' vs. 'absent' call for both Affymetrix and Agilent array analyses.
In contrast, TPS10 expression by center plants failed to attract more predator activity to the centers of populations.
In conditional knockout mice deficient in retinal retinoblastoma protein Rb, the gradient of Cre expression from center to periphery, leading to a gradual reduction of retinal thickness, was clearly visible and well topographically quantifiable.
The whole compendium of proteomics expression profiles center based on the value of the 9th decile and scale normalized [ 48- 50] using the 'normalizeBetweenArrays' normalization method available in 'limma' package [ 51] of the R suite [ 52].
If the quality of a given array does not differ greatly from the average quality in the dataset, then such subtracted expression values center around 0 and display interquantile ranges similar to other arrays.
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