The enzymatic activity ranged from 0.25 to 49.41 U/mL and 31 fungi produced more than 10 U/mL of the studied enzyme (Table 1).
The α-helical content was 21% in the case of the untreated enzyme and 5% for the freeze-dried enzyme (Table 1, see additional file 1).
In the λ phage recombination assay too, the RecBCDPs enzyme exhibited lesser efficiency than the E. coli enzyme (Table 3).
The catalytic activities of the PDE9Q453E mutant toward cGMP and cAMP were also reduced to about half of those of the wild type enzyme (Table 2).
This designed enzyme exhibited an increased rate of enzyme-mediated cyclosarin dephosphonylation of >33,000-fold relative to the wt enzyme (Table 1).
However, the apparently recombination-deficient RecBD1118ACD enzyme (Table 3) was found to be proficient in DNA repair (Fig. 6) and able to restore the growth of LCBD at low temperature (Fig. 7).
Each plasmid was digested with the appropriate restriction enzyme (Table S3) that cut the insert once, approximately in the middle in order to clone the aacC3-IV gene conferring resistance to gentamycin (GmR) [48].
The stimulation of the basal ATPase activity (kcat/Km) of this mutant by both RNA and DNA was lower than with the wt enzyme (Table 1 and Fig. 3 H), being less than 2- fold.
A linear regression analysis was performed for each methyl-sensitive restriction enzyme (Table 1).
The isolated imperatorin (4) showed the highest activity against carbohydrate metabolizing enzyme (Table 1).
The effect of metal ions shows that both lead and copper inhibited the enzyme (Table 3).
