Exact(19)
enzyme half-life.
The enzyme half-life also was better at −20 °C temperature than at 4 °C.
Enzyme half-life (t½) times at 55, 65 and 75 °C were 80, 25, and 8 min, respectively.
The inactivation profile and the enzyme half-life are shown in Fig. 7 and Table 5, respectively.
Stabilizers such as glycerol, for example, can increase the enzymatic stability (Singh et al. 1991; Kapoor and Kuhad 2008) and the enzyme half-life during storage.
At pH 8 and 70 °C, the disulfide bridge increased the enzyme half-life 20-fold in the presence of substrate.
Similar(41)
This resulted in an enzyme half life of almost 30 days.
In the presence of calcium, conversion of His-77 to Glu resulted in a 4-fold enhancement in enzyme half life and a 9 °C upward shift in T50, which was observed in compare to the wild type.
Incubation with oligonucleotide 1 had the greatest protective effect increasing the enzyme half life to >2300 seconds.
By incubating samples of the enzymes at various temperatures and measuring the activity at regular intervals, the enzyme activity half-life was calculated at three different temperatures.
The enzyme's half-life of deactivation was 13 min at 45°C.
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