Exact(18)
Msp I was used as a restriction enzyme (b).
a AGPP enzyme; b SSS enzyme; c GBSS enzyme; d SBE enzyme.
qRT-PCR for 16 kDa oleosin (A) and starch debranching enzyme (B; SDBE).
The transmembrane enzymes disulfide bond forming enzyme B (DsbB) and vitamin K epoxide reductase (VKOR) are central to oxidative protein folding in the periplasm of prokaryotes.
Fig. 6 Saccharification of pine needles by a commercial enzyme and b indigenously produced cellulase enzyme Fig. 7 Total sugars produced from Pine needles by a commercial enzyme b indigenously produced enzyme.
This effect disappeared where the reaction media was polar as the polar solvent (acetonitrile) is more effective in stripping off the water from the enzyme; (b) reduction in particle size as revealed by SEM.
Similar(42)
In production of the novel enzyme, B-512F dextransucrase was truncated at N and C terminals and fused to glutathione S-transferase.
Human PRDM5 CDS was amplified by PCR using specific primers modified at the 5' end with the sequence recognized by BamH1 restriction enzyme (B-PR5f1: 5'- GC GGATCCCTGGGCATGTACGTGCCGGA-3'- GC GGATCCCTGGGCATGTACGTGCCGGA-3AGCTACACCAT-3'- GC GGATCCCTGGGCATGTACGTGCCGGA-3of the pCDNA3-HA vector (Invitrogen).
a Free enzymes, b metabolon (enzyme clusters), c fusion enzymes, d scaffolded enzymes.
Higher ethanol-tolerance generally correlated with lower ethanol-productivity, as XI and XII produced less ethanol than X (Additional file 2B), which was due to rewired glycolysis (XI and XII), suppressed solvent formation genes (XI and XII) and inhibited co-enzyme B biosynthesis (XI).
Enzyme D shows a lower dosage response than enzymes B and C but achieves cellulose conversion levels above 90% at higher enzyme loadings.
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