Ai Feedback
Exact(35)
For an efficient hydrolysis of lignocellulosic materials a different ratio might be desirable and this may be achieved just by adding further copies of either gene in the fusion construct in order to obtain an optimized enzyme blend.
FastStart High Fidelity Enzyme Blend (Roche Diagnostics, Indianapolis, IN) was used in both rounds of PCR.
Enzyme blend mixing of fraction C15 and C20 gave higher activity than testing the two fractions individually.
A previous study was performed utilizing the same enzyme blend on alkali-pretreated sugarcane bagasse in a 120-h batch hydrolysis [ 8].
Enzymatic hydrolysis reactions were carried out by treatment with a novel commercial enzyme blend Cellic C-Tec derived from Trichoderma reesei (Novozymes A/S, Bagsværd, Denmark).
The obtained enzyme blend was concentrated using a Micron ultrafiltration unit (Millipore Corporation, Bedford, MA) for later application in sugarcane hydrolysis assays.
Similar(25)
Enzyme blends were identified, outperforming the individual commercial preparations.
This developing practice is based on the understanding that the effective degradation of the complex structure of lignocellulose requires not only cellulases, but also supplementary enzyme blends of ligninases (laccases), hemicellulases, and accessory proteins, depending on the morphological characteristics of the lignocellulosic biomass.
In Exp. 1, effects of enzyme blends (amylase, protease and xylanase) at different dose rates [high (H) vs. medium (M) vs. low (L)] on the coefficient of apparent digestibility (CAD) were investigated in vitro using a two-stage enzyme incubation method.
Synergy among enzymes from individual enzyme extracts is also another advantage to utilization of these enzyme blends [ 7, 8].
The use of enzyme blends showed the synergistic effect of the enzymes produced by these two fungi.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com