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The ratios of enzyme mixtures were also studied.
In this context, the development of optimized enzyme mixtures is of interest.
All enzymes and enzyme mixtures tested yielded protoplasts (Figure 1D and Table 1).
The most effective preparations were then taken as basic components for the formulation of enzyme mixtures.
Consequently, all enzyme mixtures decreased (P<0.001) the acetate to propionate ratio.
Moreover, among the tested enzyme mixtures, the one prepared with the mutant epCelStrep_2 led to improvements in sugar conversions.
Xyn10A is a suitable candidate to be added into the high efficient fungi enzyme mixtures for biomass conversion.
Better understanding of how to match pretreatments with improved enzyme mixtures will lead to lower costs for industrial biorefining.
However, activity of endoglucanase was reduced at 100°C compared to the single Cel5A and enzyme mixtures, which was probably a result of modified protein conformation.
On the other hand, some other research findings revealed that the application of enzyme mixtures have shown a better performance than individual enzymes presumably due to synergism [30].
Interestingly, HPLC analyses revealed that the final product yield (glucose and cellobiose) of fusion enzyme BC is improved compared to 1 1 enzyme mixtures of identical molar concentrations.
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