It was amazing how easy the cycle sequencing and the automatic sequencers were to use.
Cleaned PCR products were used as a template for cycle-sequencing reactions using BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems, Foster City, CA).
Cycle sequencing was performed following the instructions supplied along with BigDye® Terminator v3.1 Cycle Sequencing Kit.
Cycle sequencing reactions were carried using BigDye Terminator v. 3.1 Cycle Sequencing Kit (Life Technologies) following the manufacturer's instructions.
Purified cycle sequencing products were analysed on an ABI Prism 3130 xl Genetic Analyzer.
Direct cycle sequencing in both directions was performed with the same sets of primers.
Sequencing was carried out using BigDye® Terminator v3.1 Cycle sequencing kit following manufacturer instructions.
Sequencing was performed by using Big Dye terminator cycle sequencing kit (Applied BioSystems, Waltham, MA, USA).
Results: Fluorescent dye[ndash ]labeled cycle sequencing identified the constructed fusion toxin gene.
The inserts were subjected to cycle sequencing (BigDye terminator v3.1 Cycle Sequencing kit, Applied Biosystems, USA).
Cycle sequencing was performed using The BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Sweden).
