Sentence examples for cycling sequencing from inspiring English sources

Cycling sequencing conditions for forward primers were as follows: (40 cycles): 96°C for 10 sec; annealing, 50°C for 5 sec; elongation, 60°C for 4 min; 4°C hold.

Cycling sequencing conditions for forward primers were as follows: (40 cycles): 96°C for 10 s; annealing, 50°C for 5 s; elongation, 60°C for 4 min; 4°C hold.

A suspected sample was subjected to direct sequencing using the cycling sequencing system (Applied Biosystems, Foster City, CA, USA) and run on Perkin-Elmer ABI-377 automatic sequencer.

This study investigated six functional genomic polymorphisms in genes, which have different enzyme functions, or expressions, and DNA repair by PCR-RFLP assay and cycling sequencing.

After thermal cycling, sequencing reactions were processed and analyzed on an ABI-377 or an ABI-3700 capillary sequencer as described in [ 36].

After thermal cycling, sequencing reactions were processed and analyzed on ABI 3730xl, ABI-377 or ABI-3700 capillary sequencer as described in [ 78].

PCR was carried out using a cycling sequence of 95°C for 5 min followed by 35 cycles of 95°C for 1 min, 45°C for 1 min and 72°C for 2 min, which was then followed by a final extension of 72°C for 10 min. New sets of primers were designed from the sequence analysis of these virus-specific cDNAs for determining the remaining sequence.

"Big Shots" on ABC-TV moved a cycling sequence from Malibu.

Other tracks use a cycling sequence of chords as a structure for small amounts of improvisation, or written parts played in a loose, improvised manner.

Cycling sequence was performed with Big Dye Terminator Version 3.1 kit (Applied Biosystems, Foster City, CA), following manufacturer's instructions.

The approach is illustrated through several test cases, in which different combinations of objective and constraint functions, design variables and cycling sequence patterns are examined.