Results are given as percentages of fish erythrocytes containing micronuclei (total of 2000 counted cells).
Took an aliquot of the cell suspension, counted cells, and determined their viability.
Consequently, the quantitatively counted cells data reveals that the aspartate is most sensitive chemoattractant for the S. typhimurium (t tests, n = 6, mean ± SEM, *: <0.001).
For small-budded cells, we counted cells with a bud size smaller to ¾ of mother size.
For large-budded cells, we counted cells with a bud size equal or larger than ¾ of mother size.
In order to provide a quantitative measurement, we counted cells with positive staining on their apical surface.
SA-β-Gal -positive were quantified by counting stained and unstained cells and expressed as the percentage of SA-β-Gal -positive cells over the total counted cells.
The number of SA-b-gal positive cells was determined in 100 randomly chosen low-power fields (x100) and expressed as a percentage of all counted cells.
The percentage of apoptotic cells was calculated by dividing the number of TUNEL-positive cells by the number of counted cells.
Then, we counted cells double stained for c-Fos and the inhibitory neuron markers (GAD67, PV, CB and CR) in MD and control rats.
The number of counted cells was multiplied by 5 to obtain the total number of positive cells in the dentate gyrus.
