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Subsequent flowing of GOx to conjugate the antibody allowed the labeling of the captured target by the formation of a sandwich-type immunocomplex.
4) PCR amplification of captured target libraries.
The captured target DNA was then eluted with NaOH and amplified by PCR.
Co-immunoprecipitated proteins were normalized to the level of captured target protein for analysis.
Capture efficiency refers to the fraction of captured target cells relative to the total captured cells.
Finally, the captured target DNA was eluted by NaOH and amplified by PCR.
Similar(36)
The label step ligates a universal biotinylated adapter to the captured targets.
The circularized captured targets were amplified and barcoded by PCR using the library-free BSPP protocol as previously described (Diep et al., 2012).
This made use of the captured targets to calculate the transformation parameters between the scans, and the geo-referenced control points.
Once forums were identified, a selection process for posts was employed (Fig. 1) to ensure that recent entries were captured, targeting discussions on disease-related symptoms.
After the hybridization, the captured targets were selected by pulling down the biotinylated probe/target hybrids by using streptavidin-coated magnetic beads (Dynabeads MyOne Streptavidin T1, Life Technologies).
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