Sentence examples for captured targets from inspiring English sources

For each sample PCR amplification of captured targets was performed using 25 ul of Multiplex PCR Kit (Qiagen), 0.5 uM of each indexed primer, 5 ul of capture reaction and nuclease-free water to 50 ul.

While all QMC-beads revealed significant amount of captured targets, the highest binding capacity was observed for the beads prepared in the presence of 1 nmol probe: 5 × 1012 target molecules per cm2, or one captured QMC target per ~20 nm2 of the bead surface area.

The label step ligates a universal biotinylated adapter to the captured targets.

The circularized captured targets were amplified and barcoded by PCR using the library-free BSPP protocol as previously described (Diep et al., 2012).

This made use of the captured targets to calculate the transformation parameters between the scans, and the geo-referenced control points.

The captured targets were subjected to massive sequencing using the Illumina HiSeq2000 with the paired-end 100 bp read option, according to the manufacturer's instruction.

Subsequent flowing of GOx to conjugate the antibody allowed the labeling of the captured target by the formation of a sandwich-type immunocomplex.

Once forums were identified, a selection process for posts was employed (Fig. 1) to ensure that recent entries were captured, targeting discussions on disease-related symptoms.

4) PCR amplification of captured target libraries.

Capture efficiency refers to the fraction of captured target cells relative to the total captured cells.

The captured target DNA was then eluted with NaOH and amplified by PCR.