Reversible immobilization of the antibodies was performed using an anti-mouse Fc antibody covalently coupled in high density to a CM5 sensor surface according to the manufacturer's instructions (mouse antibody capture kit; GE Healthcare).
Highly purified Hpa1-His protein was collected from the 200-mM imidazole eluent and used in the experiments after the His tag was removed by treatment with the Novagen Enterokinase Cleavage Capture Kit (EMD Biosciences Inc., Darmstadt, Germany).
This, police would later say, was Garland's "capture kit".
Cells were lysed and mRNA was isolated using an mRNA Capture kit (Roche).
Thrombin cleavage was carried out using biotinylated thrombin cleavage capture kit (Novagen) following manufacturer's instructions.
Messenger RNA was isolated from control or stimulated (anti-CD3/anti-CD28 see above) lymph node cells using an mRNA capture kit (Roche) according to the manufacturer's instructions.
Sensor chip CM4, amine coupling reagents [N-ethyl-N'-dimethylaminopropyl carbodimide, EDC; N-hydroxysuccinimide, NHS; and 1 M ethanolamine HCl (pH 8.5 ], human antibody capture kit and HBS-EP+ buffer were obtained from GE Healthcare.
The GST tag was removed using a Thrombin Cleavage Capture kit (Novagen, Madison WI) and glutathione agarose batch chromatography and removal was confirmed by immunoblotting with anti-GST (Upstate, Lake Placid NY).
The observed average read depth for Agilent V2 capture kit was 30.5X, for the Agilent V3 capture kit was 80X, for the Agilent V4 capture kit was 48X, and for the Agilent V5 capture kit was 63X.
Additionally, no single exome capture kit covers every exon.
Subsequently enterokinase was removed following the recommendations of the Enterokinase Cleavage Capture Kit (Novagen).
