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The cells were stained for tartrate-resistant acid phosphatase (TRAP), a commonly accepted marker of osteoclasts [ 20], using a Sigma TRAP kit (no. 386-A, Sigma Chemicals, St . Louis MO) according to the manufacturer's instruction.
Dane Clark, on drums, switched off among a trap kit, hand drums, and a minimal stand-up kit with a foot pedal below the tom-tom.
The synthesizers simulated chamber orchestras, non-Western stringed instruments or the deep bass of a didgeridoo; two percussionists used hand drums alongside a trap kit.
The weatherproof camera trap kit is designed to survive the elements.
Naturebytes has just kicked off a Kickstarter for a Raspberry Pi-powered camera trap kit for capturing wildlife photos.
The track includes guest musicians River Tiber and Karriem Higgins who knocks out some trap kit beats with the flair of a pro player.
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Smaller samples were collected by the personnel of forest reserves in 52 localities across Madagascar using trapping kits provided by us.
The in situ detection of osteoclasts was carried out on metastatic bone tissue sections using the tartrate-resistant acid phosphatase (TRAP) activity kit assay (Sigma).
To quantify osteoclastogenesis, a tartrate-resistant acid phosphatase (TRAP) staining kit (Sigma) was used according to manufacturer's instructions in conjunction with image analysis of TRAP positive, multinucleated cells using AxioVision software (Zeiss).
Seven µm sections of the decalcified long bones were then cut with a microtome (Leica RM2125 RT; Leica Microsystems, Bannockburn, IL) and stained with hematoxylin and eosin (H&E) and TRAP staining kit (Kamiya biomedical company, Seattle, WA).
TRAP staining kit was purchased from Sigma-Aldrich (USA).
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