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Individual patient samples often harbored multiple, distinct, leukemia-associated cell populations identifiable by their surface marker expression, functional performance of signaling pathway in the face of cytokine or growth factor stimulation, as well as their response to apoptosis-inducing agents.
Tie2-expressing macrophages can be distinguished from Tie2− tumor-associated macrophages by their surface marker, Mrc1.
The latter can be distinguished from the conventional B-2 cells by their surface marker expression, anatomical localization and activation requirements.
Since uterine NK cells recruited in the steady state are less mature, as noted by their surface marker phenotype, the cytokine/hormone milieu changes the functional phenotype needed for successful pregnancy or immune protection develops in situ.
In this work we have isolated from bone marrow aspirates human mesenchymal stem cells characterized by their surface marker profile [ 1, 17] and their osteogenic, chondrogenic and adipogenic potential [ 5- 7, 22, 23] to study the expression profile of genes involved during maturation and adipogenic differentiation.
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Cells were purified with a MoFlo cell sorter (Beckman Coulter) by their surface markers.
Double-negative (DN, CD4−CD8−) thymocytes go through four developmental stages (DN1 to DN4), which are distinguishable by their surface markers.
By evaluating their surface marker pattern and expression of iNOS and TNF we could proof that the IFNβ producing cells were a subpopulation of Tip-DCs with T cell priming abilities of professional APCs.
We differentiated ES cells and sampled developing cells at different time points by purifying them according to their surface marker profile using fluorescence activated cell sorting (FACS) as outlined in Figure 1A and Supplementary Figure 1A.
The AML-initiating cells, defined by their cell surface marker expression (CD34+,CD38-), were able to proliferate, disseminate, and maintain an AML phenotype similar to the patients they were derived from after transplantation into severe combined immune-deficient (SCID) mice [53].
The DN4 subpopulation were defined by their cell surface marker expression and subsequently sorted using a Vantage cell sorter (Becton Dickinson) or AutoMacs magnetic cell sorter (Miltenyi Biotec).
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by their surface roughness
by their morphology marker
by their surface texture
by their surface composition
by their surface sugar
by their surface receptor
by their surface charge
by their surface chemistry
by their surface plasmon
by their surface signature
by their surface expression
by their surface structure
by their surface phenotype
by their surface water
by their surface modification
by their surface functionalization
by their surface area
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