by the dideoxy method

USAGE SUMMARY

The phrase "by the dideoxy method" is not correct in English; it appears to contain a typographical error.
If you meant "by the dideoxy method," it can be used in scientific contexts, particularly in molecular biology or genetics, to refer to a specific technique for DNA sequencing. Example: "The DNA was sequenced by the dideoxy method to ensure accuracy in the results."

⚠ May contain grammatical issues

Science

Human-verified examples from authoritative sources

Exact Expressions

6 human-written examples

Three clones were sequenced 3 times in both orientations by the dideoxy method, using M13 universal primers and ET Dynamic Terminator for MegaBACE (GE Healthcare, Fairfield, CT, USA).

Emerging Infectious Diseases

The nucleotide sequences of NA and HA DNAs were confirmed by the dideoxy method using ABI PRISM 377 DNA Sequencer (Applied Biosystems).

BMC Infectious Diseases

We have chosen to analyze ΦX174 due to the extant experimental evolution literature and methodology with this organism as well as its status as the first genome sequenced by the dideoxy method of Sanger et al. (1977 , 1978.

Genome Biology and Evolution

cDNA was subcloned in pBluescript vector (Stratagene) and DNA sequence analysis was performed in both strands by the dideoxy method using T7 DNA polymerase (Amersham Biosciences) and 5'-[α-S]-labeled dATP (NEN Life Science Prod. Zaventem, Belgium).

BMC Genomics

Purified DNA was directly sequenced by the dideoxy method by using a BigDye Terminator v3.0 Ready Reaction Cycle Sequencing Kit and was analyzed in an ABI PRISM 3700 sequencer (each from Applied Biosystems, Foster City, CA, USA).

Emerging Infectious Diseases

The amplified products of blaOXA-2-related and blaPSE-1-related genes of several of the samples were sequenced by the dideoxy method by using fluorescent terminators and an automatic laser fluorescent DNA sequencer (Pharmacia Biotech, Uppsala, Sweden) (Table) (6 ).

Emerging Infectious Diseases

Human-verified similar examples from authoritative sources

Similar Expressions

54 human-written examples

DNA sequencing was carried out by using the dideoxy method, and sequences were compared with previously published sequences from the global collection of serotype A strains (10 ).

Emerging Infectious Diseases

The PCR was performed by an initial 5 min denaturation at 95°C followed by 40 cycles of 95°C for 30 s, 58°C 62°C for 35 s and 72°C for 45 s with a final elongation at 72°C for 10 min. Sequencing was performed using the dideoxy method by fluorescent automated sequencing on the ABI 3130 xl genetic analyzer (Applied Biosystems, Foster City, CA, USA).

Human Molecular Genetics

Sanger and his coworkers used the dideoxy method to sequence human mitochondrial DNA.

Encyclopedia Britannica

At least 10 individual molecular clones obtained using the TOPO-TA cloning kit (Invitrogen, Cergy Pontoise, France) were sequenced in both directions by the dideoxy chaintermination method (Big Dye Terminators v.3.1, Applied Biosystems, Paris, France) on an 3130xl Genetic Analyzer (Applied Biosystems).

Plosone

Products were cloned in pGEM-T vector (Promega) and manually sequenced by the dideoxy sequencing method.

BMC Plant Biology

Expert writing Tips

Best practice

Consider rephrasing as "using the dideoxy method" or "via dideoxy sequencing" for improved clarity and grammatical correctness. Double-check your phrasing to ensure it aligns with standard scientific writing conventions.

Common error

Avoid adding unnecessary words. Using a more direct phrasing like "dideoxy sequencing" or "Sanger sequencing" often improves clarity.

Linguistic Context

The phrase functions as a prepositional phrase indicating the method used to perform DNA sequencing. While grammatically questionable as stated by Ludwig AI, it aims to specify the approach taken in scientific experiments. Refer to the examples for context.

Expression frequency: Uncommon

Frequent in

Science

100%

Less common in

News & Media

0%

Formal & Business

0%

Encyclopedias

0%

Ludwig's WRAP-UP

The phrase "by the dideoxy method" is used to describe a DNA sequencing technique, but Ludwig AI flags it as grammatically questionable. While the phrase appears in several scientific publications, its correctness is debatable. The analysis reveals that the phrase is most commonly found within scientific contexts. While prevalent in technical writing, consider using clearer alternatives such as "using the Sanger sequencing method" or "via dideoxy sequencing" to enhance clarity and adhere to standard grammatical conventions. Despite its presence in credible sources, attention to phrasing can improve the overall quality and precision of scientific communication.

More alternative expressions

Phrases that express similar concepts, ordered by semantic similarity:

using the Sanger sequencing method

This alternative specifies Sanger sequencing, a more common and recognizable term for the dideoxy method.

via dideoxy sequencing

Changes the preposition for a slightly more concise phrasing while retaining the core meaning.

employing the dideoxy chain termination method

Replaces "method" with "chain termination method", providing a more specific term for the sequencing process.

sequenced according to the dideoxy method

Reorders the sentence to emphasize the sequencing action and its accordance with the specified method.

utilizing the dideoxy technique

Substitutes "method" with "technique", offering a slight variation in terminology.

based on the dideoxy principle

Focuses on the underlying scientific principle of the dideoxy method rather than the method itself.

with dideoxy chain termination

This simplifies the sentence structure and focuses on the key action of dideoxy chain termination.

dideoxy sequencing was performed

This alternative puts the focus on dideoxy sequencing itself.

the Sanger technique was applied

This replace to the original technique name, referring to Sanger.

sequence determination by dideoxy chemistry

This focuses on the chemical aspect of the sequencing process.

FAQs

What is the correct way to refer to the Sanger sequencing method?

The method is accurately called the Sanger sequencing method or the "dideoxy method". Both are appropriate in scientific writing.

Is it better to say "Sanger sequencing" or "dideoxy sequencing"?

"Sanger sequencing" is more widely recognized. However, "dideoxy sequencing" is also correct and refers to the same process.

How does Sanger sequencing relate to the dideoxy method?

Sanger sequencing and the "dideoxy method" are synonymous; Sanger developed the dideoxy chain-termination method for DNA sequencing.

What are some alternative ways to describe DNA sequencing using the Sanger method?

You can use phrases like "using the Sanger sequencing method", "via dideoxy sequencing", or "employing the dideoxy chain termination method depending on the context.