Sentence examples for dideoxy method from inspiring English sources
The phrase "dideoxy method" is not correct in written English; it should be "dideoxy sequencing method." You can use it when discussing a specific technique in molecular biology for DNA sequencing.
Example: "The dideoxy sequencing method has revolutionized our ability to read genetic information."
Alternatives: "dideoxy sequencing" or "Sanger sequencing method.".
Sanger and his coworkers used the dideoxy method to sequence human mitochondrial DNA.
So-called first-generation sequencing technologies, which emerged in the 1970s, included the Maxam-Gilbert method, discovered by and named for American molecular biologists Allan M. Maxam and Walter Gilbert, and the Sanger method (or dideoxy method), discovered by English biochemist Frederick Sanger.
Nuclear amplified fragments of three individuals for each population were sequenced in both directions using a modification of the Sanger dideoxy method as implemented in a double stranded DNA cycle sequencing system with fluorescent dyes.
The PCR product from each of the 96 genotypes (two parents as well as a subset of 94 individuals from F2 population) was purified and sequenced directly using Sanger's dideoxy method on an automated capillary-based DNA sequencer (ABI 3730xl, Applied Biosystem, USA) in both forward and reverse directions as per standard ABI sequencing protocol.
DNA sequencing using the Sanger dideoxy method was performed by Polymorphic DNA Technologies Alamedaa, CA, USA).
Three clones were sequenced 3 times in both orientations by the dideoxy method, using M13 universal primers and ET Dynamic Terminator for MegaBACE (GE Healthcare, Fairfield, CT, USA).
The resulted products were sequenced by a dideoxy termination method using an applied Biosystems 373 DNA sequencer (LGC Genomics GmbH, Germany) in the two directions (forward and reverse) by the same PCR-used primers.
At least 10 individual molecular clones obtained using the TOPO-TA cloning kit (Invitrogen, Cergy Pontoise, France) were sequenced in both directions by the dideoxy chaintermination method (Big Dye Terminators v.3.1, Applied Biosystems, Paris, France) on an 3130xl Genetic Analyzer (Applied Biosystems).
Amplified DNA fragments were purified and sequenced with the Sanger dideoxy terminator method [ 47].
All sequencing was completed using the Sanger dideoxy sequencing method at the Australian Genome Research Facility AGRFF) in Brisbane, Australia.
Products were cloned in pGEM-T vector (Promega) and manually sequenced by the dideoxy sequencing method.
