using the Sanger sequencing method

USAGE SUMMARY

The phrase "using the Sanger sequencing method" is correct and usable in written English.
It can be used when discussing a specific technique for DNA sequencing in scientific or technical contexts. Example: "The researchers conducted their experiments using the Sanger sequencing method to accurately determine the genetic sequences."

✓ Grammatically correct

Science

Human-verified examples from authoritative sources

Exact Expressions

8 human-written examples

In our previous study, a total of 3, 111 cDNA clones derived from a B. chinense root cDNA library were 5' single-pass-sequenced using the Sanger sequencing method [ 19].

BMC Genomics

The insert of isolated plasmid was sequenced using the Sanger sequencing method (Eurofins Genomics).

BMC Genomics

We further sequenced the PCR products for all 50 loci amplified from the two parents using the Sanger sequencing method to confirm these SNPs.

BMC Genomics

Double-end cDNA sequencing was carried out by the Genome Sciences Centre at the British Columbia Cancer Agency (Vancouver, BC, Canada) using the Sanger sequencing method.

BMC Genomics

We further sequenced PCR products of all 44 loci amplified from the two parents using the Sanger sequencing method to further confirm these SNPs.

BMC Genomics

Using the Sanger sequencing method, the total number of unique transcripts from these studies was low (less than 3,000 genes) and they were not able to represent the expression levels of the original samples.

BMC Genomics

Human-verified similar examples from authoritative sources

Similar Expressions

52 human-written examples

High-quality BAC sequences were determined by the shotgun method using the Sanger sequencing protocol, as described previously.

DNA Research

SSR validation was performed using RT-PCR and the Sanger sequencing method.

BMC Plant Biology

The monoclones were selected randomly, and the inserts present sequenced based on the M13F primer by the Sanger sequencing method using ABI 3700 sequencer under the control of Sequencing Analysis 5.2.0 with the default parameters.

BMC Genomics

A similar rate of chimera formation was found with the Sanger sequencing method using cloned libraries derived from MDA reactions [ 9]. 31.8% of clones (having an average of 3 kb inserts) were chimeric giving a frequency of about one rearrangement per 10 kb of MDA product.

BMC Biotechnology

Of the 670 primer pairs newly designed from the radish unigene sequences (http://radish.plantbiology.msu.edu), single DNA fragments were amplified by 528 primer pairs; of which, 351 showed nucleotide polymorphism between 'Sayatori' and 'Aokubi', which are the parents of F2 plants used for DNA marker mapping, by the Sanger sequencing method.

DNA Research

Expert writing Tips

Best practice

When describing experimental procedures, use "using the Sanger sequencing method" to clearly indicate the specific technique employed for DNA sequencing. This promotes clarity and reproducibility.

Common error

Avoid simply stating that sequencing was performed without specifying the method when accuracy and detail are necessary. Stating "sequencing was performed" lacks the specificity of "using the Sanger sequencing method", which is crucial in scientific contexts.

Linguistic Context

The phrase "using the Sanger sequencing method" functions as a descriptive element, specifying the technique employed in a process or experiment. As Ludwig AI indicates, this phrase is correct and usable in written English, particularly in scientific contexts. The phrase often clarifies the methodology section of a research paper.

Expression frequency: Uncommon

Frequent in

Science

100%

Less common in

News & Media

0%

Formal & Business

0%

Encyclopedias

0%

Ludwig's WRAP-UP

In summary, "using the Sanger sequencing method" is a grammatically correct and appropriate phrase for scientific and technical writing, as confirmed by Ludwig AI. It's primarily used to specify the DNA sequencing technique in research methodologies, ensuring clarity and reproducibility. While less common in general contexts, it's highly relevant in scientific domains. Related alternatives include "employing Sanger sequencing" and "Sanger sequencing was utilized". When writing, remember to specify the sequencing method for accuracy, especially in scientific publications.

More alternative expressions

Phrases that express similar concepts, ordered by semantic similarity:

employing Sanger sequencing

Replaces "using the method" with a more concise verb, maintaining the focus on the Sanger sequencing technique.

implementing the Sanger sequencing technique

Emphasizes the implementation aspect of the Sanger sequencing technique.

applying Sanger sequencing

Similar to "employing", it offers a synonym that maintains the focus on the sequencing process.

the Sanger sequencing approach was applied

Uses the term "approach" instead of "method", providing a slight variation in terminology.

Sanger sequencing was utilized

Changes the sentence structure to passive voice, emphasizing the sequencing process rather than the act of using it.

Sanger sequencing was performed

Similar to utilizing, this alternative also put more emphasis on sequencing process.

Sanger's method was used for sequencing

It rephrases Sanger sequencing to Sanger's method and slightly changes the focus.

Sanger-based sequencing was conducted

Uses a more formal tone by replacing "using" with "was conducted" and emphasizes Sanger's contribution

with Sanger sequencing

Focuses on Sanger sequencing as a tool or instrument being with something.

sequence determination via Sanger method

This alternative is more technical and concise, suitable for scientific writing where brevity is valued.

FAQs

What is the purpose of "using the Sanger sequencing method" in scientific writing?

In scientific writing, "using the Sanger sequencing method" specifies the exact DNA sequencing technique employed, ensuring clarity and enabling reproducibility of the research.

Are there alternatives to "using the Sanger sequencing method"?

Yes, you can use alternatives like "employing Sanger sequencing" or "Sanger sequencing was utilized", depending on the context.

Is "using the Sanger sequencing method" formal or informal?

The phrase "using the Sanger sequencing method" is generally considered formal and appropriate for scientific and technical writing. It is precise and unambiguous.

When is it appropriate to use "using the Sanger sequencing method"?

Use "using the Sanger sequencing method" when you need to be specific about the DNA sequencing technique used in your research. It is essential for clarity and reproducibility.