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Infectious NDV could be generated in the cells infected with recombinant vaccinia virus, which expressed T7 RNA polymerase, by simultaneous expression of antigenome-sense NDV RNA from the full-length plasmid and NDV NP, P, and L proteins from cotransfected plasmids.
Moreover, transfection of p120-catenin to MEF cells stabilized the ectopically expressed E-cadherin (CFP-E-cadherin, corresponding to the 145 kDa band) or the endogenous protein (corresponding to the 120 kDa band) and totally prevented the decrease in the levels of these proteins caused by simultaneous expression of pPS1 (Figure 5C, compare lanes 3 and 5).
Enhanced ROS scavenging by simultaneous expression of cytosolic MeCu/ZnSOD and peroxisomal MeCAT1 in transgenic cassava also confirmed the improved tolerance towards drought and cold temperatures [ 7].
We performed rescue experiments with SKN-BE2c by simultaneous expression of siMMP-2 and TLX by western blot (Supplementary Figure 1).
This includes the phenotypic flexibility of the TMC in the blastocoel, as exemplified by simultaneous expression of GAD and the serotonin receptor protein in the same cell.
In this study, using three-dimensional cultures, migration and invasion assays, genetic tools and bioinformatic analysis, we have defined the genetic landscape induced by simultaneous expression of HER2 and HER3 in mammary cells.
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The advantages of this method are that miRNAs are efficiently transcribed and processed by the cell's own machinery, and expression can be monitored by the simultaneous expression of EGFP which (unlike conventional expression reporter vectors) is expressed only if the miRNA insert is correctly spliced.
In these cells, rHu BChE was expressed as mostly tetrameric form by the simultaneous expression of proline-rich attachment domain.
Over time, the survival of these photoreceptor cells may be impaired by the simultaneous expression of the aberrant transcripts or mutant proteins, if expressed.
KIM-1 is expressed predominantly in renal tubules, which, judging by the simultaneous expression of markers of dedifferentiation [ 10], are currently under the influence of agents that cause injury, but is absent from completely atrophic tubules.
The corresponding recombinant baculoviruses BAC-Gp5M and BAC-Gp234E were employed to produce two types of VLPs: basic Gp5M VLPs, by the simultaneous expression of Gp5 and M proteins; and complete VLPs, by the co-expression of the six PRRS proteins after co-infection.
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