Sentence examples for by serial transfer of from inspiring English sources

Serial 0.33-log dilutions were achieved by serial transfer of 43 µl into 50 µl diluent (final volume = 93 µl) from the top to the bottom of the plate.

Larvae for Western blots, weight and Q-PCR were pre-laid for three days, and then were staged by serial transfer of parent flies (10 mating pairs per vial) at 12 hour intervals.

Plasmodium berghei ANKA (PbA) was maintained in vivo by serial transfer of parasitized erythrocytes (PE) from infected to naïve mice.

In Figure 1(a), we plot the change in specific growth rate during the evolution experiment without the addition of ethanol, which was carried out by serial transfer of an aliquot of culture to the fresh M9 medium every 24 hours starting from E. coli wild-type strain W3110.

Spiegelman and coworkers put genomic RNA of bacteriophage Qβ into conditions that were perfect for replication (excess of active replicase and substrates), and then, applied various selective pressures, the simplest one being limitation of the time available for replication by serial transfer of aliquots of the reaction mix to new test tubes.

The recombinant population was then adapted by serial transfer on IJ434, rapidly resolving the now polymorphic mutations from the adaptation.

The most promising strain, expressing the XI mined from Prevotella ruminicola TC2-24, was further adapted for aerobic and fermentative growth by serial transfers of D-xylose cultures under aerobic, and followed by microaerobic conditions.

The membranes were then taken through an ethanol dehydration series (once each on 30% and 50% ethanol followed by twice on 70% ethanol) by serial transfer to filter paper soaked in the solvents followed by a final transfer to chloroform.

Recombinant populations were typically propagated by serial transfer for 5 hr, allowing lysis before each transfer.

The recombinant pool was grown by serial transfer for nearly 30 generations.

After several hundred hours of chemostat growth, the second phase began with a recombination between the final chemostat population (T7 LME_1940) and the starting phage (T7 LME_200); the recombinant pool was grown by serial transfer for another 60 generations to yield T7 LME_2000.