Sentence examples for by serial passaging of from inspiring English sources

Expansion is achieved by serial passaging of the adherent cell population.

RM1 cells were obtained from Dr. T Thompson, Baylor College, and RM1 BM) was developed by serial passaging of RM1 in vivo [13].

All BE cell lines were adapted to serum-free conditions in keratinocyte serum-free (KSF) medium supplemented with bovine pituitary extract and epidermal growth factor (EGF) (Invitrogen Corp. Carlsbad, CA) by serial passaging of cell lines into successively lower concentrations of serum until the cell lines survived without serum present.

MEFs were immortalized by serial passages of primary MEFs.

It is beyond the scope of our objective to study self-renewal by serial passage of stroma from the "ossicles".

This live- attenuated vaccine, originally derived by serial passages of a virulent strain of M. bovis, has been used to prevent TB since 1921.

Gene rearrangements can also be generated in cell culture by serial passages of RV at high multiplicity of infection (MOI) [10] [14].

Sp2cko/cko MEFs and corresponding wild type and heterozygous MEFs (Sp2wt/cko) were immortalized by serial passages of primary MEFs until they pass their growth-crisis stage.

As an alternative approach, we inoculated into the same group of SCID mice (N = 8) a diverse mixture of B. hermsii serotypes that we had prepared by serial passage of relapse serotypes into immunocompetent mice (see methods for details).

The attenuated virus, MP-12, developed by serial passage of the wt RVFV ZH548 strain in the presence of 5-fluorouracil [27], carries A at M847 and encodes Glu277, suggesting that the presence of Glu277 in Gn does not always make RVFV virulent.

The parasites were maintained by serial passage of blood from infected mice to non-infected ones on weekly basis.