Sentence examples for by sequencing a single from inspiring English sources

Similarly, a 0.1 kb gap in CCMP452 lacking shotgun clones was spanned by sequencing a single PCR product.

Normalization efficiency was then assessed by sequencing a single pass 5' read of each cDNA insert using BigDye Terminator ver3.1 sequencing chemistry.

The finding of a large number of transcripts without matches among the Solanaceae species will complicate the efforts of establishing a single reference genome for the Solanaceae by sequencing a single representative species.

So, if multiple contig sequences are obtained by sequencing a single species' genome, or if metagenomic sequences are phylogenetically classified into groups using some classification methods, genes on the sequences can be more precisely predicted by the MGA.

To analyze these heterozygous sites, for each wAnaITG genome we attempted to phase the variation by sequencing a single F1 offspring of each wAnaITG containing strain (while absent of wAnaINF) of D. ananassae that had been crossed to a strain lacking both integrated and infectious forms of wAna (see Materials and Methods for details).

The 83 Paris samples were genotyped for the single nucleotide polymorphism rs3822086 by sequencing in a single direction using the BigDye Terminator Cycle Sequencing kit v3.1 (Applied Biosystems, Forster City, CA, USA).

The Ga mutation was identified by sequencing crtD; a single C → T base change at position 259 alters CAG to a TAG stop codon.

Furthermore, point mutation of PBRM1 and the other chromatin-modifying genes was originally identified by sequencing of a single biopsy from each of several ccRCC.

Moreover, the volume of sequence data acquired by environmental sequencing is several orders of magnitude higher than that acquired by sequencing of a single genome [19].

Most of them were fully sequenced by a single round of Sanger sequencing starting from 75 bp downstream the start codon of yEGFP.

10, 11 To achieve similar results with Sanger sequencing, it would be necessary to perform 12 sequencing reactions per sample, totaling 144 reactions (considering that each amplicon can be sequenced by a single reaction).