Sentence examples for by sequencing a second from inspiring English sources

Thirty-five candidates were tested by sequencing a second allele using previously published complementation data [ 19].

Each mutation was confirmed by sequencing a second independent PCR reaction.

Singletons were confirmed by sequencing a second individual from the same colony when available.

b let-596 (h782) and let-630 (h355) both confirmed by sequencing a second allele and failed to complement each other.

All sequencing reactions were performed in both forward and reverse directions, and all mutations were confirmed by sequencing a second independent PCR product.

The primers used for sequencing are detailed in Table 1 and any mutation detected was verified by sequencing a second PCR product to rule out artefacts introduced by Taq error.

Because this TCGA cohort is not enriched in early-onset cases but rather reflects the overall age distribution of serous ovarian cancer, we decided to complement the INHBA and INHA mutation screening by Sanger sequencing a second series of 62 other French early-onset ovarian epithelial cases (<40 years), without detectable BRCA mutation.

Each fragment containing mutations was amplified by PCR and sequenced a second time to confirm that the identified mutations were not due to PCR artifacts.

By sequencing the first ophioglossoid fern and a second horsetail (E.

Both the known and the novel miRNAs were confirmed by sequencing of a second small RNA library constructed the same way as the one used in the first sequencing.

All mutations were confirmed (and the possibility of mutation artifacts was ruled out) by sequencing of a second, separately amplified PCR product.