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These data suggest that the total Symbiodinium sequence diversity (not distribution) present in shallow water M. capitata in Kāne'ohe Bay can be recovered with either sequencing a few clones from many coral colonies or by sequencing a large number of clones from a few coral colonies.
Phylogenetic evidence regarding transmission can often be improved by sequencing a large number of virus genomes from each infected individual.
We have partially made up for this lack of genomic information by sequencing a large number of cDNAs.
Each individual PCR product is a copy of one mtDNA molecule and by sequencing a large number of clones; the mutation load per base pair can be quantified.
In this study, we extended previous alfalfa SNP discovery experiments by sequencing a large number of genotypes including elite genotypes from four major US alfalfa breeding companies.
The first dedicated effort in the public domain identified SNPs by sequencing a large number of amplicons from prescreened single copy loci using the traditional Sanger sequencing approach.
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Technical variability might be reduced by sequencing a larger number of samples and excluding poor-quality samples from the analysis39,40, but this adds considerably to the expense of what ideally would be a first-stage screening procedure to identify biomarker candidates that would then be validated by quantitative rtPCR.
Additionally, we may have improved identification by sequencing a larger region of the tuf gene.
"The authors have accomplished an amazing technical feat by sequencing such a large fraction of an extinct genome," says geneticist David Reich of Harvard Medical School in Boston.
For the genomewide association study, a RAD catalog was constructed using a subset of individuals (6 12 total individuals from 3 to 6 collections) and then used to align and perform genotyping by sequencing on a larger sample of individuals (N = 518) from 21 collections distributed throughout the Pacific Northwest range of Pacific lamprey (Hess et al. 2013).
To determine the identities of ChIP DNA by sequencing methods, large numbers of sequence reads are required.
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