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Exact(5)
For adenine and thymine, both strands can be used like in re-sequencing experiments.
The strand with the less stable 5" hydrogen bonding is usually selected as the mature miRNA, although both strands can be functional.
If the lifetime of the polymerase is long enough, both strands can be sequenced multiple times (called "passes") in a single CLR.
Sometimes both strands can be detected, in which case the miRNA* designates the less predominant form of the mature miRNA [ 15, 16].
This phenomenon is consistent with results from other species (e.g., human) that both strands can be transcribed (Werner et al. 2009).
Similar(55)
A unit of replication which has both strands catalytic is never observed in the model, possibly because both its strands can be exploited by parasites as well as other (non symmetric) units of replication.
Further, both DNA strands can be analyzed in parallel, and the specific target amplification can be validated simultaneously with mass analysis, providing a level of internal confirmation not achievable by other methods.
Previous studies have demonstrated that both of the dsRNA strands can be loaded into the RNA-induced silencing complex (RISC) and that both strands can facilitate RNAi [ 54- 56].
Locating the regions of the mt genomes associated with the replication origins (ORs, also known as control regions or A+T rich regions) for both "+" and "-" strands can be challenging based on a knowledge of the nucleotide sequence of mt genomes alone.
But such strands can be made by the hand of man.
Frequently you also got a sense of how inseparable those various strands can be in his work.
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