Sentence examples for blocking solution and applied from inspiring English sources
Secondary antibodies were diluted 1∶1000 in blocking solution and applied for one hour, at room temperature in the dark.
Anti-mitochondrion specific heat shock protein 70 (mtHSP70) antibody (clone JG1, Abcam, www.abcam.com) and anti-á-tubulin (clone YOL 1/34, Abcam) antibody were diluted 1 250 in the blocking solution and applied to the specimens on ice overnight.
Antibodies were diluted in blocking solution and applied overnight at 4°C on a nutator.
The antibody was diluted 1 200 in blocking solution and applied to deparaffinized slides, and the slides were incubated at 4 °C overnight.
Mouse monoclonal antibodies MF20 against sarcomeric myosin heavy chain and S46 against atrial myosin heavy chain were obtained from the Developmental Studies Hybridoma Bank, diluted 1 10 in blocking solution, and applied to embryos overnight at 4°C.
Primary antibodies (rat anti-BrdU, AbD Serotec, Oxford, UK, 1/300; mouse anti-NeuN, Chemicon, Cork, Ireland, 1/500 or mouse anti-BrdU, BD Bioscience, Oxford, UK, 1/100) were diluted in blocking solution and applied overnight at 4 °C.
The RR sera and control sera with unrelated autoantibody activity were diluted 1/100 in the blocking solution and separately applied to the membrane, incubated for 2 hr at room temperature, washed in three changes of TBST and then in TBS to remove the detergent.
The primary antibody directed against CD31 was diluted 1 : 800 in protein-blocking solution and applied to the sections, which were incubated overnight at 4°C.
The primary antibodies directed against CD31 and PCNA were diluted 1 : 100 and 1 : 50, respectively, in protein-blocking solution and applied to the sections, which were then incubated overnight at 4°C.
Membranes were then washed with blocking solution, and appropriate secondary antibodies diluted in blocking solution were then applied for 1 h at room temperature.
After blocking the tissue with M.O.M. blocking solution and 5% normal human serum (NHS) for 60 min at RT, the antibody complex was applied to the tissues and incubated for 60 min at RT.
