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Exact(7)
P-values for phenotypic coefficients were calculated with a two-sided t-test using cor.test function in R. A linkage map consisting of 179 SSR markers (Fig. 4) covering the 12 chromosomes with a total length of 1849.4 cM (an average length of 9.4 cM) between two markers was constructed as described by Jiang et al. (2004).
On each chromosome, 1,000 marker loci were randomly located and each segment between two markers was considered to harbor a potential QTL, giving 5,000 markers and 4,995 potential QTL in total.
The average interval distance between two markers was shorter in POP-1 (3.25 cM) than the POP-2 (4.57 cM).
The selected markers had minor allele frequencies (MAF) > 0.1 and were close to equidistant over the region, so that the average distance between two markers was 0.1 cM.
The total number of COs between two markers was divided by the number of individuals in the BC and the result was divided by the length of the interval between the two markers to generate the RF in cM/Mb.
The number of markers per linkage group varied from 22 to 127, with an average of 71 markers per group, and the average distance between two markers was 4.1 cM.
Similar(53)
Indeed, despite the median distance of two neighboring markers being 103 kb, the median recombination rate between two markers is 5.7% (Figure 2B), much smaller than the rate of ∼1 recombination per 100 kb per meiosis in Sc [21].
Most of the spaces between two markers were smaller than 20 cM on the genetic linkage map.
The complete map size was 407.3 cM, with an average marker spacing of 5.7 cM and the largest distance between two markers being 17.6 cM.
If r 2 between two markers is equal in two populations, but their corresponding r has opposite sign, the gametic phase is reversed [ 16].
The average spanning distance between two markers is 1.95 cM in our study, which is the lowest value published to date for a RADSeq study.
Related(20)
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