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The maximum difference between two SSRs was 100 bp.
The maximum distance between two SSRs was specified as 100 bases.
The mean separation between two SSRs was ~10.6 kb, equivalent to one SSR per 29 sequences.
The search criteria were: di-nucleotide repeats ≥6, tri-nucleotide to hexa-nucleotide repeats ≥5, and the largest interval between two SSRs was ≤100 bases.
However, the average distance between two SSRs was quite similar between unigenes and genomic sequences for common bean, soybean, Medicago, and lotus (Table 5).
Similar(55)
Three SSRs were found in the shotgun scaffolds, but no BAC containing these SSRs was found.
The remaining four SSRs were identified in intron regions.
Ten SSRs were scored as indels as well.
For compound SSRs the maximum distance between two SSR runs was 50 bp.
The relative ratio between two SSR PPs' concentrations was more important than the absolute quantities, which is consistent with previous results [ 34].
The search was restricted to motifs having at least 14 bp length (i. e., di-nucleotide > 7; tri-nucleotide > 5; tetra-nucleotide > 4; penta-nucleotide > 3 and hexa-nucleotide > 3) and the minimum difference between two SSRs in a sequence (interruption) was 100 bases.
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