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Our design allows a good optical coupling efficiency between sample and detector.
In this experiment, two pairs of slits positioned between sample and detector were used, which provided the typical wave vector resolution in the vertical scattering plane of about 0.003 nm-1.
The small pixel size also alleviates the use of higher energies (or smaller distances between sample and detector).
The distance between sample and detector was about 160 nm, which formally corresponds to a scattering event of 2 θ ∼ 50° at λ = 0.82656 Å and ensures that the magnetite peaks of interest (220 400, at 2 θ ∼ 16−22.7°) can be collected with high intensity (low geometric spread), but still well separated from the directly transmitted beam.
According to the small-angle approximation the FOV per single diffraction pattern depends on the wavelength λ, the distance between sample and detector X and the pixel size of the detector D, i.e. The smaller pixel size thus allows a large field of view (here ∼8 × 8 µm), which in principle opens the possibility for large beam experiments.
Since the two contrast mechanisms work in different ways for different specimens and for different parts of the same specimens, the only solution is empirical preliminary tests to find the optimum distance between sample and detector and the corresponding optimum level of staining.
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This issue could be overcome with the use of secondary collimators (positioned between the sample and detector) to more accurately select the scattering angles of photons which reach the detector.
The distance between the sample and detector was 350 mm.
A wire grid IR polarizer, inserted between the sample and detector, was used as an analyzer in the imaging experiments to polarize the light between 0° and 90°.
If the distance between source and sample is Z 0, sample and detector is Z, the defocusing distance Zd and the magnification factor M are then defined as,, respectively.
AUC values also exhibited an excellent correlation (R = 0.996) between manual sampling and detector measurements with a mean difference of 9.3% ± 9.7% (max 24.1%, min −3.2%).
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