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We used the hypothesis test P(H1) that represents the probability of the alternate hypothesis that the difference between sample and control groups is due only to chance.
To find peaks with differential intensity between sample and control, threshold levels p-value<0.05 and absolute (log ratio)≥1 were used in each pair.
The 2(−ΔΔCT) method [100] was used to quantify the difference of mRNA expression of each gene with respect to a housekeeping gene (ΔCT) between sample and control (ΔΔCT).
T-test (unpaired; two-tailed; equal variances) and log ratio values were calculated for each peak between sample and control independently for all three sample/control pairs: HG vs. control, HG+rVEGF vs. control, and HG+nitric oxide donor vs. control.
Significant differences in crosslinking frequencies between sample and control template are candidate regulatory DNA contacts [ 4].
As a result, a 40 50% difference can be seen between sample and control solutions, unambiguously showing the fact of generation of hydroxyl radicals during redox cycling.
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Difference between samples and control were evaluated using GraphPad Prism 5.0 software (GraphPad Software Inc., USA).
Competitive hybridizations were carried out on oligonucleotide arrays; processing of the expression data generated ratio intensities between samples and control, with positive values corresponding to genes more expressed in myofibers.
The ΔΔCt method was used to compare relative fold changes between samples and control.
We narrowed down our analysis to four cases that showed significant repression of the targeted gene and performed differential expression analysis between samples and control gRNA using DESeq.
In addition, RE digestion and bisulfite conversion are sources of variability as the initial treatment of DNA can cause non-specific template degradation and confound the relative comparison between samples and control 100% methylated DNA [ 39].
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between sample and questionnaire
between diabetic and control
between command and control
between privacy and control
between mapping and control
between intervention and control
between sample and model
between sample and polishing
between identification and control
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