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To maximize the efficiency of the Direct PCR, the ratio between sample and dilution buffer was optimized.
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cDNA concentrations were normalized between samples and serial dilutions were used as template for PCR amplification.
An in-line sampling and dilution system for droplet aerosols from medical nebulisers is described.
You should also factor in sample cell rinse and dry time between different proteins, which will add 2 min. Spinning samples prior to data collection requires 10 min at 14,000 rpm, but sample spinning and dilution can be done simultaneously with data collection.
Improving the Sensitivity of Bacteria Detection and Quantification in Urine Samples via Sample Dilution and Filtration.
Sample stability and dilution integrity were also tested and verified.
Interestingly, aberrant k-mers were not fully overlapping between sample dilutions, suggesting that low quality is derived from both sample preparation and system noise.
Samples filtration and dilution was done according with ISO 11466/1999.
We find that the estimated coefficients on the interaction term between ROE and DILUTION is insignificant for the whole sample.
The interaction between determination method and sample dilution was more significant than other two-way interactions.
Serum samples were diluted in sample dilution buffer (1 200 dilutions for bovine samples and 1 2,000 dilutions for sheep/goat samples).
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