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These mRNAs should be translated at a low level since they have fewer ribosomes attached to them, and in fact their TE and RPKMs are lower.
Proteins that can fold spontaneously into their functional conformation are free from that constraint and can be translated at a higher speed.
To question whether such a strategy could be translated at a pre-clinical level for myelin diseases, we investigated whether ectopic expression of PSA-NCAM would be of benefit to promote remyelination by adult primate Schwann cells in the context of CNS demyelination.
To estimate how many of the novel extensions we detected might be translated at a biologically significant level, we estimated a threshold for biological significance as the fifth percentile of readthrough rates for the phylogenetically conserved extensions that were translated in the D. melanogaster embryo, a rate of 1.2%.
Proteins that require chaperones have to be translated at a speed that fits the time required for chaperone recruitment (i.e., chaperone abundance and turnover rate), otherwise the protein will fold spontaneously into the wrong conformation, thereby forming aggregates that hinder the cell viability (Geiler-Samerotte et al. 2011).
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Marketing materials may not be translated at all or they're badly adapted to the western market.
From the transcribed bicistronic mRNA, the selection marker is translated at a low frequency, and the protein of interest at a high frequency.
We aimed to evaluate if such alterations are translated at a functional mitochondrial level to establish the etiopathology and potential therapeutic targets for this obstetric complication.
At the relatively earlier time of viral infection (3.5 hpi in this study) when the viral RNA amount is not high enough, EV71 RNA is translated at a higher efficiency than most of the cellular mRNAs.
Thus, medical research, for example, is regulated by the Helsinki Declaration at the international level (Helsinki Declaration 2012), which is translated (at a technical level) into the work of individual doctors and medical researchers primarily through contract, or for example, through the Clinical Trials Regulation of the EU.
The reasons could be that not all of the transcribed genes are translated at a certain time point or that the dynamic range of protein amount reaching up to 12 and more orders of magnitude is not covered by the currently available proteomic technologies.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com