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According to the literature [16], [17], activation of Clb5 first causes Clb2 to be transcribed at a low level, which is sufficient to activate Clb2's own transcription factors, Mcm1/SFF (represented as Mcm1 in the model), which then cause Clb2 to be transcribed at a higher level.
Since lhrA appears to be transcribed at a relatively high and constant level throughout growth, we hypothesized that the growth phase dependent accumulation of LhrA observed in Northern blots [15], [23] is likely to be the result of a post-transcriptional control mechanism.
We have assumed a low constitutive expression such that only a single mRNA molecule can be transcribed at a time.
Most amino acid biosynthetic genes are not organized in operons in A. variabilis, so one gene can be transcribed at a very low level, while all other genes in the pathway are transcribed at significant levels.
Mid-log cells were used for RNASeq analysis, thus it is possible that the other 40 genes which did not show transcript/expression may be transcribed at a different growth stage.
According to the study by Chen, et al.[ 22], the activation of Mcm1 causes Swi5 to be transcribed at a low level, and then Swi5 is activated at a higher level after Cdc20 is activated.
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Four genes (act26, act27, act32, act33) might not be transcribed at all, might be expressed with only minute activities, or are transcribed for a short time only at distinct developmental stages.
The researchers found that they could identify groups of genes with related functions by comparing their modification patterns and whether they were being transcribed at a particular time.
This indicates that the VEGF gene is transcribed at a basal level.
In the dogfish S. canicula pectoral fins, Shh, which is transcribed at a late stage in fin development, was expressed at the same time as Hoxd13 (Fig. 1I).
For BM, dnaK, vjbR, omp25, htrA and gntR were transcribed at a very low level in vitro (TSB 7.0), but were highly activated immediately in macrophage, indicating these genes play important role in Brucella intracellular survival.
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