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Here, we show that expression cassettes conferring bimodal expression can be efficiently transduced with the help of lentiviral vectors.

As shown in Fig. 2B, not only FL-derived cDC but also FL-derived pDC could be efficiently transduced with lentiviral vectors, regardless of their integration proficiencies.

In contrast, HeLa cervical cancer cells, 3T3 mouse fibroblasts and primary mouse hepatocytes were not permissive for transduction with the selected capsid variants while they could be efficiently transduced with wild-type AAV transduction (data not shown), suggesting target specificity of the selected clones and supporting their use for targeting breast cancer cells in vivo.

However, they can be efficiently transduced with lentiviral vector to overexpress CBS together with EGFP, a fluorescent reporter.

Having shown that exfoliated cells from voided urine can be used to establish short-term cultures and that these cultures can be efficiently transduced with adenovirus, we generated an adenovirus in which GFP expression was under control of the survivin promoter.

All of the samples were efficiently transduced with Ad.CMV.GFP.

Almost 100% of the cells were efficiently transduced with AdEGFP at MOI of 5,000 DNA particles/cell 48 h after infection.

In addition, the spinal cord was efficiently transduced with this strategy, showing a high neuronal tropism as assessed by the staining for the motoneuron marker choline acetyltransferase (ChAT) (Supplementary Figure 1a).

To determine if BRD4 regulates pluripotency in vivo, ESCs were efficiently transduced with either nontargeting control (NTC) or sh Brd4 lentiviral vectors and injected subcutaneously into immunodeficient (Rag2−/−) mice.

These findings suggest that even though there is no specific receptor for oxidants, their signaling may be efficiently transduced through interactions with the EGFR.

We report that such cells can be efficiently transduced by a recombinant adenoviral vector carrying the human β-glucuronidase cDNA (Ad/CMV*β-glu) and that the transduced astrocytes produce large amounts of the enzyme.