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Conclusion: Perfused human placenta can be efficiently transfected with adenoviral vectors, and the expression of the transgene can be detected in the trophoblastic cells.

Here, we show that rat pigment epithelial cells can be efficiently transfected with the PEDF-expressing non-viral hyperactive Sleeping Beauty transposon system delivered in a form free of antibiotic resistance marker miniplasmids.

Both cell lines and primary cells can be efficiently transfected with ACes and express the transgene.

Immortalized STHdhQ7/Q7 and STHdhQ111/Q111 striatal cells, which are established from WT and Q111 knock-in HD mouse embryos, respectively [ 27], are easy to culture and can be efficiently transfected with exogenous genes.

These cells were efficiently transfected with a uniform set of electric parameters for a combined HV/LV pulse (Table 1).

HeLa cells were chosen as a model human cell type because they have been used extensively for anthrax toxin entry studies and they are efficiently transfected with siRNAs [9], [16], [17].

As we predicted, the cells recruited on Ad-SiNWS were efficiently transfected with pEGFP and showed high GFP fluorescence signals.

Here, we analysed G2-phase, Artemis-deficient primary human fibroblasts that were efficiently transfected with Artemis expression constructs.

After 12 h, ECs were efficiently transfected with anti-miR-125a-5p anti-miR-125a-5p anti-miR-125a-5p anti-miR-125a-5p25a-5p repressior pre-miR-125a-5p pre-miR-125a-5p pre-miR-125a-5p pre-miR-125a-5p

Consistent with our previous report (Kawasaki et al., 2012), GFP fluorescence was clearly visible on the brain surface (Fig. 1A, arrowhead), and numerous GFP-positive cells were observed in the cerebral cortex (Fig. 1B), indicating that cortical cells were efficiently transfected with GFP using our in utero electroporation procedure in ferrets.

The results measured by a flow cytometry showed the decoy ODN could be efficiently transfected into A549 cells with a dose-dependent manner after incubating for 6 h (Fig. 1B).