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A larger difference in correlation coefficients is observed when the probe conditions are grouped based on probe level.
In addition to a comparison of methods, some suggested diagnostics based on probe level tests are also presented.
It should be clear that a probe set level normalization does not apply to methods based on probe level tests of significance.
The combined p and median t methods are both based on probe level tests of significance and seem to rank genes similarly in many cases.
We acknowledge that, in all likelihood, testing methods based on probe set summary values would have exceeded the performance of the methods based on probe level tests had a probe set level normalization been performed for the Golden Spike data.
The combined p and median t methods are both based on probe level tests of significance and perform well based on ranking genes in order of evidence of differential expression.
Similar(54)
Note that for models based on probe-level data (ANOSVA probe, FIRMA and differential expression), the intensities of all probes in the probeset (1 4) are shown.
113 chips (71 parous, 42 nulliparous) satisfied quality control thresholds based on standard Affymetrix quality control measures and graphical criteria based on probe-level model (PLM) analysis as implemented in the Bioconductor affyPLM package.
Based on our LRpath results, we identified additional circadian genes, DRD1 (FDR < 9.9×10-7), CASP1 (FDR < 0.002), PTGDS (FDR < 4.8×10-23), and PGLYRP1 (FDR < 8.5×10-7) as hypermethylated in breast tumor samples (significance levels based on probe-level LIMMA analysis, see Methods); these genes play a role in the regulation and disruption of circadian rhythm (Additional file 2: Figure S9).
In O2EM, significant differential expression is summarized according to gene annotation based on probe sets, genes, transcripts or protein levels as it is selected by the user on the tool's entry page.
Hierarchical clustering was performed on profiles based on probe status.
Related(20)
based on skill level
based on probe signal
based on water level
based on probe exon
based on probe annotation
based on probe orientation
based on probe labeling
based on probe length
based on probe sonication
based on asset level
based on transcript level
based on probe status
based on probe drug
based on probe sequence
based on payroll level
based on ability level
based on phylum level
based on education level
based on probe affinity
based on voltage level
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