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The comparative analysis detailed here was based on probe annotation and aspects of the relevant underlying biology.
DMRs were identified using the Probe Lasso algorithm [ 26], which applies a dynamic window based on probe annotation and density to record neighboring significant CpG sites and determine discrete DMR boundaries.
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Since the selection of primers and probes in this study was based on probe annotations, slight differences in the location of probes or primers on the target sequence might result in the detection of different types of splice variants on the different technologies.
In O2EM, significant differential expression is summarized according to gene annotation based on probe sets, genes, transcripts or protein levels as it is selected by the user on the tool's entry page.
Additional annotations for the Affymetrix mouse 430_2 array based on probe exon mapping, signal intensity and uniqueness on the mouse genome have also been included (7).
Expressions for genes were mean-aggregated for each gene based on Affymetrix probes annotation.
Of these candidates there were a total of 13027 probes in common among three different tissues, including those with designated gene names, those with only descriptive annotation, and those destined unknowns due to lack of homolog to any genes of other animal species based on the original probe annotation [ 11].
Many similarities can be identified between the responses against powdery and downy mildew in V. vinifera based on the annotation of probes on the chips, although a complete and detailed comparison cannot be carried out because different array platforms were used in each case.
To facilitate comparison, we generated a cross-platform probe map based on gene annotation (Methods).
Based on annotation databases, the probes have been pre-manufactured to be reverse complementary to RNA sequences, so that the labeled product could hybridize with them.
To identify any commonality of highlighted genes within biological pathways, pathway analysis using Ingenuity Pathway Analysis (IPA) was completed for the 155 genes closest to the CpG probes (based on Illumina provided annotation) that were associated with disease status based on a liberal threshold of p < 0.0001.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com