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The N 1s spectra were fitted with the Voigt function (1.2 and 0.25 eV Gaussian and Lorentzian widths, respectively) as well as background subtraction using the Shirley method.

Raw data were analysed without background subtraction using the oligo package from Bioconductor (http://www.bioconductor.org).

The volume of each protein band was extracted after background subtraction using the rolling ball method.

To perform the analysis, both the Calcein AM and Hoechst 33342 channels were blurred using a Gaussian filter followed by background subtraction using the sliding paraboloid method.

When using the threestep procedure we chose a background subtraction using the Ideal mismatch [ 14], quantile normalization [ 17], and Tukey's Biweight [ 18] method for summarization.

Relative activity and hTR content were normalized to the input or bound sample for TPP1 purification of WT TERT, after bound hTR background subtraction using the purification without tagged TPP1 OB-fold domain.

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For the brain analysis background subtraction used the "normexp" method in LIMMA with an offset of 50, otherwise the treatment of data was the same as for the gonad experiment.

The more recent version of this algorithm GCRMA performs background subtraction using information on the probe sequence composition through the calculation of binding affinities [ 6].

Therefore, detecting real fish by background subtraction using a static model or using the most recent frames as the background model is not effective.

The data was analysed using the GEArrayAnalzer Software (Version 1.0) with background subtraction (using plasmid DNA PUC18 as negative control) and normalisation with the housekeeping gene Ppia (cyclophlinA).

The interfibrillar Bragg spacing was calculated from the position of the innermost equatorial reflection after background subtraction (using a similarly obtained background pattern from the Mylar windows of the empty specimen holder) and division of fibril transform [20], [21].

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